Common Laboratory Solutions and Methods
Ethidium bromide solutions for gel staining
Stock solution is 10 mg/mL and is made with deionized water. Store the stock solution at 4°C.
Add 4 µL of the stock solution to 100 mL of dissolved agarose.
DNA Sample Loading Buffer
There are several solutions that can be used.
1) 0.25 (w/v) % bromophenol blue; 0.25% (w/v) xylene cyanol; 30% glycerol.
Make a 10 mL solution and store at either 4°C or -20°C.
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This is a common solution used in molecular biology and is the abbreviation for a 10 mM Tris, 1 mM EDTA solution. The pH of the solution is usually 8.0.
0.5 M EDTA
Add 186.1 g of disodium EDTA (always double check molecular weight and calculations) to 800 mL of dH2O. Adjust the pH to 8.0 with NaOH (ca. 20 g of NaOH pellets). Adjust volume to 1 L. Dispense into aliquots and autoclave.
Isopropylthio-ß-D-galactoside (m.w. = 238.3). Make a solution of IPTG by dissolving 2 g of IPTG in 8 mL of dH2O. Adjust volume to 10 mL and sterilize by filtration through a 0.22 µ disposable filter. Dispense the solution in 1 mL aliquots and store them at -20° C.