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    • F/2 Culture Media
    • DNA EXTRACTION FROM MARINE PHYTOPLANKTON
    • DNA EXTRACTION FROM MARINE ALGAE AND SEAGRASSES
    • RNA EXTRACTION FROM DIATOMS USING THE RNEASY KIT
    • Modified RNA Extraction Using Glass Bead Beating and the RNeasy Kit
    • RNA Extraction from Red Algae
    • REVERSE TRANSCRIPTION (RT) OF DIATOM RNA USING QIAGEN’S QUANTITECT
    • RECIPES FOR MINI-PAGE GELS
    • Transfer to nitrocellulose and alkaline phosphatase detection (BioRad)
    • Transfer to nitrocellulose and alkaline phosphatase detection (OWL)
    • Siver Stain Protocol
    • BRADFORD MICROASSAY
    • PURIFICATION OF HIS-TAGGED LUCIFERASE
    • ALGAL PIGMENTS
    • Counting Cells Using a Hemacytometer
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Home › Protocols

Protocols

Molecular Biology

  • DNA extraction from marine phytoplankton
  • DNA extraction from marine algae and seagrasses
  • RNA extraction from diatoms using RNeasy kit
  • RNA extraction from diatoms using glass beads and RNeasy Kit
  • RNA extraction from red algae
  • cDNA synthesis using Qiagene’s Quantitech RT kit
  • Cloning PCR products

Protein Analysis

  • SDS-PAGE
  • Western Blotting
    • Transfer to PVDF
    • Transfer to nitrocellulose and alkaline phosphatase detection (BioRad)
    • Transfer to nitrocellulose and alkaline phosphatase detection (OWL)
    • Chemiluminescent detection
    • Staining blots to detect proteins
      • Fast Green
      • Coomassie Blue
  • Silver Stain
  • BioRad Bradford Assay
  • Purification of His-tagged Proteins

Physiological/Biochemical Methods

  • Photosynthesis
  • F/2 Media
  • Pigments
  • Cell counting using a hemacytometer
  • Enzyme Assays

General Information

  • Common Laboratory Solutions
  • Chemical Concepts

 

Contact Info

Deborah Robertson
Professor of Biology
Warren Litsky '45 Endowed Chair in Biology
Clark University
Worcester, Mass. 01610
Lasry Center for Biosciences
224 (lab) 231 (office)

508.793.7398
debrobertson@clarku.edu
curriculum vitae (pdf)

Updates

  • Tiny Creatures Form Massive, Bright Ring Around Antarctica – Eos
  • AL-145 Purpald
  • The tardigrade genome has been sequenced, and it has the most foreign DNA of any animal
  • Climate change threatens dozens of the region’s marine species, a new study reports – The Boston Globe
  • Microscopic vampire amoebas are swarming everywhere

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