Protocols

Molecular Biology

• DNA extraction from marine phytoplankton
• DNA extraction from marine algae and seagrasses
• RNA extraction from diatoms using RNeasy kit
• RNA extraction from diatoms using glass beads and RNeasy Kit
• RNA extraction from red algae
• cDNA synthesis using Qiagene’s Quantitech RT kit
• Cloning PCR products

Protein Analysis

• SDS-PAGE
• Western Blotting
  • Transfer to PVDF
  • Transfer to nitrocellulose and alkaline phosphatase detection (BioRad)
  • Transfer to nitrocellulose and alkaline phosphatase detection (OWL)
  • Chemiluminescent detection
  • Staining blots to detect proteins
    • Fast Green
    • Coomassie Blue
• Silver Stain
• BioRad Bradford Assay
• Purification of His-tagged Proteins
  

Physiological/Biochemical Methods

• Photosynthesis
• F/2 Media
• Pigments
• Cell counting using a hemacytometer
• Enzyme Assays

General Information

• Common Laboratory Solutions
• Chemical Concepts