RECIPES FOR MINI-PAGE GELS

TYPE OF GEL: DISCONTINUOUS SDS-PAGE USING OWL PENGUIN SYSTEM
RESOLVING GEL: (variable %T, 0.375 M Tris-HCl, pH 8.8)
Volumes are in mL unless noted; total volume is 10 mL, just enough for two mini-gels.  You may choose to make 15 mL.
Final Acrylamide Percentage
Stock Solution 5 7.5 10 12.5 15 17.5 20
Acrylamide-bisacrylamide 1.25 1.88 2.50 3.13 3.75 4.38 5.00
(40% T, 3.33% C)
Distilled water 6.10 5.47 4.85 4.22 3.60 2.97 2.35
10% SDS 0.1 0.1 0.1 0.1 0.1 0.1 0.1
1.5 M Tris-HCl, pH 8.8 2.5 2.5 2.5 2.5 2.5 2.5 2.5
10% (w/v) Ammonium persulfate 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL
(make fresh regularly)
Temed 5 µL 5 µL 5 µL 5 µL 5 µL 5 µL 5 µL
(after degassing)
STACKING GEL: (4%T, 0.125 M Tris-HCl, pH 6.8)
Volumes are in mL unless noted; total volume is 10 mL enough for two mini-gels
Stock Solution 10 mL 5 mL 2.5 mL
Acrylamide-bisacrylamide 1 0.5 0.25
(40% T, 3.33% C)
Distilled water 6.35 3.17 1.59
0.5 M Tris-HCl, pH 6.8 2.50 1.25 0.63
10% SDS 0.1 0.05 0.025
10% (w/v) Ammonium persulfate 50 µL 25 µL 12.5 µL
Temed 10 µL 5 µL 2.5 µL