Protocols
Molecular Biology
- DNA extraction from marine phytoplankton
- DNA extraction from marine algae and seagrasses
- RNA extraction from diatoms using RNeasy kit
- RNA extraction from diatoms using glass beads and RNeasy Kit
- RNA extraction from red algae
- cDNA synthesis using Qiagene’s Quantitech RT kit
- Cloning PCR products
Protein Analysis
- SDS-PAGE
- Western Blotting
- Transfer to PVDF
- Transfer to nitrocellulose and alkaline phosphatase detection (BioRad)
- Transfer to nitrocellulose and alkaline phosphatase detection (OWL)
- Chemiluminescent detection
- Staining blots to detect proteins
- Fast Green
- Coomassie Blue
- Silver Stain
- BioRad Bradford Assay
- Purification of His-tagged Proteins
Physiological/Biochemical Methods
- Photosynthesis
- F/2 Media
- Pigments
- Cell counting using a hemacytometer
- Enzyme Assays
General Information