Below are some current projects from the McCullough lab:
Sexual selection across the invaded range of exotic dung beetles
Biological invasions are a pervasive feature of the Anthropocene that provide unique opportunities to study the process of rapid evolutionary change. Current invasion studies are aimed at understanding the eco-evolutionary factors that influence invasion dynamics, but one potent evolutionary factor remains curiously unexplored: sexual selection. Variation in sexual selection can affect the success, spread, and speed of biological invasions by promoting adaptation and species persistence in stressful or altered environments, or affecting life-history tradeoffs between reproduction and dispersal. However, the details of how sexual selection varies across the range of exotic species remain unknown. We are studying the impact of sexual selection on invasion dynamics, using the recently introduced, agriculturally important dung beetle Onthophagus taurus as a model system. 
Behavioral ecology of Onthophagus orpheus
The tunneling species Onthophagus orpheus is a beautiful, iridescent beetle that is native to forested areas of the eastern United States. Conveniently, it can be collected in large numbers from pitfall traps baited with dog poop at Clark’s Hadwen Arboretum – just half a mile from campus. Large males have a large thoracic horn, small males have a tiny thoracic horn, and females are hornless. But we know essentially nothing else about its natural history! We are conducting behavioral observations to learn about their fighting, sneaking, courtship, and parental care behaviors. 
Form and function of animal weapons
Animal weapons differ widely among species, but the drivers of this diversity remain poorly understood. Horns, antlers, and claws are “tools” that males use to pinch, pry, and strike opponents in combat, so the most intuitive explanation for weapon diversity is that it reflects structural adaptations to different fighting styles. That is, differences in the way males fight, or in where they fight, may favor corresponding changes in weapon form. Because weapons are used as tools, they can experience substantial stresses and strains during fights that ultimately cause them to break. We are studying the costs and consequences of weapon damage across the animal kingdom, and how the likelihood of breakage influences the evolution of fighting behavior within and among species. We also study the scaling relationships between weapon size and body size, and how these allometric patterns depend on the relative importance of physical fighting versus aggressive signaling. 
Reproductive physiology and the evolution of ejaculate-female interactions
Sexual selection in general, and post-mating sexual selection in particular, is a powerful engine of speciation due to the coevolution of male and female reproductive traits. In species with internal fertilization, successful reproduction depends on highly coordinated interactions between male and female reproductive proteins that occur within the female reproductive tract. These proteins are among the most rapidly evolving proteins in the animal kingdom, so coevolution between male and female reproductive proteins is expected to drive specific molecular interactions within species and reproductive incompatibilities between species. However, the nature of these interactions is understudied. In many species, sperm are also stored within the female’s reproductive tract for extended periods of time before being used for fertilization, and molecular contributions from females are essential for maintaining sperm motility and metabolism. Our research uses quantitative proteomics to study these ejaculate-female interactions and their importance in maintaining species boundaries and mediating reproductive outcomes.