{"id":473,"date":"2025-10-17T15:49:47","date_gmt":"2025-10-17T15:49:47","guid":{"rendered":"https:\/\/wordpress.clarku.edu\/jdresch\/?page_id=473"},"modified":"2025-10-20T20:05:37","modified_gmt":"2025-10-20T20:05:37","slug":"biophysical-analysis-of-tf-protein-dna-interactions","status":"publish","type":"page","link":"https:\/\/wordpress.clarku.edu\/jdresch\/?page_id=473","title":{"rendered":"Biophysical analysis of TF protein-DNA interactions"},"content":{"rendered":"<p style=\"font-weight: 400\">Homeodomain transcription factors (HD TFs) are a large class of evolutionarily conserved DNA binding proteins that contain a basic 60-amino acid region required for binding to specific DNA sites. In&nbsp;<em>Drosophila<\/em> <em>melanogaster<\/em>, many of these HD TFs are expressed in the early embryo and control transcription of target genes in development through their interaction with&nbsp;cis-regulatory modules. Our collaborators from the Drewell and Spratt labs have developed a streamlined expression and purification protocol to produce large yields of&nbsp;<em>Drosophila<\/em>&nbsp;HD TFs. These purified HD TFs are currently being used in biophysical experiments to structurally and biochemically characterize the ability of these HD TFs to bind to different DNA sequences.&nbsp; Our lab is utilizing bioinformatic and statistical tools to analyze this data and further probe how nucleotide differences contribute to TF-DNA specificity in the HD family.<\/p>\n<p>&nbsp;<\/p>\n<p style=\"font-weight: 400\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-477\" src=\"https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-scaled.jpg\" alt=\"\" width=\"1878\" height=\"2560\" srcset=\"https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-scaled.jpg 1878w, https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-220x300.jpg 220w, https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-751x1024.jpg 751w, https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-734x1000.jpg 734w, https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-768x1047.jpg 768w, https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-1127x1536.jpg 1127w, https:\/\/wordpress.clarku.edu\/jdresch\/wp-content\/uploads\/sites\/383\/2025\/10\/FTZEVE-1503x2048.jpg 1503w\" sizes=\"auto, (max-width: 1878px) 100vw, 1878px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Homeodomain transcription factors (HD TFs) are a large class of evolutionarily conserved DNA binding proteins that contain a basic 60-amino acid region required for binding to specific DNA sites. In&nbsp;Drosophila melanogaster, many of these HD TFs are expressed in the &hellip; <a href=\"https:\/\/wordpress.clarku.edu\/jdresch\/?page_id=473\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":571,"featured_media":0,"parent":29,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-473","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=\/wp\/v2\/pages\/473","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=\/wp\/v2\/users\/571"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=473"}],"version-history":[{"count":0,"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=\/wp\/v2\/pages\/473\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=\/wp\/v2\/pages\/29"}],"wp:attachment":[{"href":"https:\/\/wordpress.clarku.edu\/jdresch\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=473"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}