{"id":39,"date":"2013-06-07T15:19:11","date_gmt":"2013-06-07T15:19:11","guid":{"rendered":"https:\/\/wordpress.clarku.edu\/fisherville\/?page_id=39"},"modified":"2014-08-04T15:30:59","modified_gmt":"2014-08-04T15:30:59","slug":"updates","status":"publish","type":"page","link":"https:\/\/wordpress.clarku.edu\/fisherville\/updates\/","title":{"rendered":"News"},"content":{"rendered":"<header><em>August 4, 2014<\/em><\/header>\n<header><strong>National Park Service Project Proposal Submitted<\/strong><\/header>\n<header>\u00a0<\/header>\n<header><\/header>\n<header><\/header>\n<header><\/header>\n<header><\/header>\n<header><\/header>\n<header>This past week The Fisherville Redevelopment Company teamed up with the Town of Grafton to submit a proposal to National Park Service\u2019s (NPS) Rivers, Trails and Conservation Assistance (RTCA) Program.\u00a0 The program seeks to help increase people\u2019s access to recreational activities, waterways and to promote conservation projects.\u00a0 Many of NPS\u2019s goals, therefore, align with projects that have previously been proposed at the Livings Systems Laboratory, Mill Villages Park, and the rest of the Fisherville Mill Site.\u00a0 The proposal that was submitted seeks the assistance of NPS in the design and construction of trails, increased river access, art exhibits and educational curriculum in the Fisherville Mill Site.\u00a0 If awarded, the NPS would send personnel to Grafton to team up with other partners to offer their expertise and coordination in the development of these projects.\u00a0 As always, community participation would be a project priority.<\/header>\n<header>\u00a0<\/header>\n<header>\u00a0To see examples of the previous RTCA projects in MA, <a href=\"http:\/\/www.nps.gov\/orgs\/rtca\/loader.cfm?csModule=security\/getfile&amp;pageid=641563\">click here<\/a>.<\/header>\n<header>\u00a0<\/header>\n<header>As for now, we\u2019ll keep our fingers crossed, and keep you posted with updates on this site!<\/header>\n<header>\u00a0<\/header>\n<header><\/header>\n<header><em>July 18, 2014<\/em><br \/>\n<strong>A Visit from The URI\u00a0Coastal\u00a0Institute Senior Fellows<\/strong><\/header>\n<header>\u00a0<\/header>\n<header><\/header>\n<header>On Thursday, July 17<sup>th<\/sup>, about 25 researchers from the University of Rhode Island Coastal Institute Senior Fellows Program stopped by the Living System\u2019s Lab for a quick tour of ecological design in action.<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-324 aligncenter\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-42-300x225.jpg\" alt=\"photo 4\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-42-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-42-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-42-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>The group includes leaders from NGOs, researchers and policy makers from URI and elsewhere that are committed to the sustainable use and management of coastal ecosystems.\u00a0 They came to the LSL as a part of a trip to learn about the local innovative efforts to improve the health of our natural resources.\u00a0 Gene led the tour of the canal restorers and the greenhouse, and answered the thoughtful questions of the intrigued participants related to ecosystem biodiversity and the tank species self-selection process.\u00a0 Several people of the group were participating in remediation projects already, and were eager to learn of the techniques implemented on the site.\u00a0 One participant said she was even considering the implementation of canal restorer technology in a damaged watershed in Indonesia.<\/p>\n<p style=\"text-align: left\">\u00a0<a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-2-2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-322 alignleft\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-2-2-300x225.jpg\" alt=\"photo 2 (2)\" width=\"255\" height=\"194\" \/><\/a> <a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-23.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-323 alignnone\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-23-300x225.jpg\" alt=\"photo 2\" width=\"253\" height=\"193\" \/><\/a><\/p>\n<p style=\"text-align: left\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-52.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-325 aligncenter\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-52-300x225.jpg\" alt=\"photo 5\" width=\"253\" height=\"192\" \/><\/a><\/p>\n<p style=\"text-align: left\">For more information on the URI Coastal Institute Senior Fellows, visit their site: \u00a0http:\/\/web.uri.edu\/coastalinstitute\/fellows\/senior-fellows\/<\/p>\n<p style=\"text-align: left\">\u00a0<em>June 17, 2014<br \/>\n<\/em><strong>How to Build an Eco Machine<\/strong><\/p>\n<p style=\"text-align: left\">On May 2<sup>nd<\/sup> and 3<sup>rd<\/sup>, John Todd Ecological Design, Inc hosted its first Ecological Design and Aquatic Restorer Construction Workshop at our very own Living Systems Lab.\u00a0 Participants came together from different fields and locales to learn about the basics of ecological design and began to explore some of the social, political and economical challenges of these types of projects.\u00a0 On the second day, the participants had a chance to get their hands dirty and have a crack at designing and building aquatic cells\u00a0and canal restorers using a new method.\u00a0 In addition to adding local plants, they also planted kale to investigate the potential for aquatic cell\u00a0agriculture in the greenhouse.\u00a0 One month later, the plants seem to be taking extremely well.\u00a0 Scroll down to see pictures of the new design methods as well as the most recent photos.<\/p>\n<\/header>\n<header>\n<p style=\"text-align: center\"><em><strong>Aquatic Cell\u00a0Design<\/strong><\/em><\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-22.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-311\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-22-300x225.jpg\" alt=\"photo 2\" width=\"233\" height=\"177\" \/><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-312 alignnone\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-12-300x225.jpg\" alt=\"photo 1\" width=\"235\" height=\"179\" \/><\/a>\u00a0<a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-41.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-304 size-medium\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-4-e1403189591777-225x300.jpg\" alt=\"photo 4\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-4-e1403189591777-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-4-e1403189591777-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-308 size-medium\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-31-e1403189553114-225x300.jpg\" alt=\"photo 3\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-31-e1403189553114-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-31-e1403189553114-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-309\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-41-300x225.jpg\" alt=\"photo 4\" width=\"254\" height=\"193\" \/><\/a>\u00a0\u00a0<a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-5.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-305\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-5-300x225.jpg\" alt=\"photo 5\" width=\"250\" height=\"190\" \/><\/a><\/p>\n<p style=\"text-align: center\"><em><strong>New Canal Restorer<\/strong><\/em><\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-11.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-306\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-11-300x225.jpg\" alt=\"photo 1\" width=\"262\" height=\"200\" \/><\/a> <a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-21.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-307\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-21-300x225.jpg\" alt=\"photo 2\" width=\"264\" height=\"201\" \/><\/a> <a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-302\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-2-300x225.jpg\" alt=\"photo 2\" width=\"262\" height=\"199\" \/><\/a> <a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-3.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-303\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/photo-3-300x225.jpg\" alt=\"photo 3\" width=\"261\" height=\"198\" \/><\/a><\/p>\n<p style=\"text-align: left\">If you are interested in learning more about Ecological Design, there will be another workshop offered in Grafton August 14<sup>th<\/sup> and 15th.\u00a0 Students, community members and people from all professional backgrounds are welcome.\u00a0 Follow this link to the John Todd Ecological Design website to register and for more information.<\/p>\n<p><em>http:\/\/www.toddecological.com\/news\/workshop.php<\/em><\/p>\n<h1><\/h1>\n<h1><em>June 11,2013<\/em><\/h1>\n<p><strong>Our first visit of the summer<\/strong><\/header>\n<div>Last week Professor Hibbett, and two undergraduate research assistants (myself and Sam Kovaka), visited the site. It was a beautiful sunny day! We met Gene Bernat there, and he gave us a tour of the greenhouse. He updated us on the status of the project. We have a lot to do! Soon we will inoculate the mycelium beds in the greenhouse. We found mushrooms growing at the banks of the canal where the previous wood chips were laid out. We also spotted some turtles swimming in the canal! &#8211; Vanessa <em>June 15, 2013 <\/em><strong>Plans<\/strong> After our meeting at Clark; Gene, Jim Rice, Brian Seitzman, myself and David set goals and plans for the project. Our biggest challenge was that of deciding on a name. An important goal for the project was to establish it as a teaching platform for education and outreach. We heard about Jim&#8217;s passive sampler study and his plans to start this summer. Jim told all of us about the time frame he had planned for the testing, deployment of 10-12 samplers in July, and the analysis in August. Brian also told us about his project,using the site as a business model for economic sustainability. He wishes to see the project as a path to generate income as well and integration into the local economy. <strong>Inoculating<\/strong> June 25, 2013 David and I moved forward with our plans for the site to reinoculate the mycoreactors. Our first step was to culture the sample we got from Paul Stamets through Gene. I made some agar plates and cultured the three species (Irpex, T.V.G, and Pleurotus). We made some media out of rye grain in small mason jars and milk jugs. We autoclaved them and then I inoculated three jars with different volumes of water (20 ml, 30 ml, 40 ml) and 1 milk jug for each species. We inoculated one extra jug for pleurotus so that we could grow some oyster mushrooms for food on coffee grounds from Acoustic Java (a local coffee shop near campus). The mycelium has been growing in the plates, jars, and jugs. Everything is going well! &#8211; Vanessa<\/p>\n<div id=\"attachment_227\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0622.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-227\" class=\"size-medium wp-image-227\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0622-300x225.jpg\" alt=\"jugs, jars, and plates\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0622-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0622-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0622-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-227\" class=\"wp-caption-text\">jugs, jars, and plates<\/p><\/div>\n<div id=\"attachment_228\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0632.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-228\" class=\"size-medium wp-image-228\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0632-300x225.jpg\" alt=\"mycelium growing!\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0632-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0632-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0632-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-228\" class=\"wp-caption-text\">mycelium growing!<\/p><\/div>\n<div id=\"attachment_229\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0621.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-229\" class=\"size-medium wp-image-229\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0621-300x225.jpg\" alt=\"In the hood\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0621-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0621-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0621-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-229\" class=\"wp-caption-text\">In the hood<\/p><\/div>\n<p><strong>More Plans<\/strong> 06\/27\/2013 Today Professor Hibbett and I developed more concrete plans for the Fisherville mycoreactors. We shook the mycelium jugs and jars (this was after a week) some of them, mostly the Irpex, had really taken over the rye grain and shaking them loose was hard. We realized there was a lot that needed to be done, quickly!<\/p>\n<ul>\n<li>subculturing the pleurotus Professor Hibbett brought on to new plates<\/li>\n<li>Autoclaving bags on Monday with rye grain and coffee grounds (from Acoustic Java).<\/li>\n<li>Inoculation on Tuesday<\/li>\n<\/ul>\n<p>We also realized the water volume in some of the jars was too dry so we need to increase it. &#8211; Vanessa <strong>Subculturing and Increasing Water Volumes<\/strong> 06\/28\/2013 Today was the start of our new plans! I autoclaved some water and then added it to the jars of Pleurotus and T. R.G. I added 10ml to each jar. I did this under hood using sterile technique. I also subcultured the Whole Foods Pleurotus onto 4 new MEA plates. &#8211; Vanessa<\/p>\n<div id=\"attachment_235\" style=\"width: 235px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0635.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-235\" class=\"size-medium wp-image-235\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0635-225x300.jpg\" alt=\"Subcultured Pleurotus plates in the hood.\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0635-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0635-768x1024.jpg 768w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0635.jpg 960w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><p id=\"caption-attachment-235\" class=\"wp-caption-text\">Subcultured Pleurotus plates in the hood.<\/p><\/div>\n<p><strong>Some Inoculation<\/strong> 07\/01\/2013 Today I filled 8 bags with rye grain and 5 with coffee grounds. For the rye the ratio was 1L of rye and 900 ml of water per bag. For the coffee the ratio was 2L of coffee grounds and 250ml of water per bag. One bag had 1L and no water added, and another bag had 2L and no water added either. I autoclaved these bags and left them under the hood to cool overnight. &#8211; Vanessa <strong>The Big Inoculation Day<\/strong> 07\/02\/2013 Today we inoculated 20 bags of rye with the Irpex, Pleurotus, and Trametes culture we had growing in jugs and jars. The ratio remained 1L of rye to 900 ml of water. It was a very big day because I could only autoclave 12 bags at a time, before they were ready (sterile and cool enough) to inoculate. I used the bag sealer that Gene sent us to seal them. unfortunately 2 bags ripped in the autoclave and I had to throw them out. Nevertheless, the day was a success! -Vanessa <strong>The Bags<\/strong> 07\/17\/2013 After being away for some time I checked on the bags and they&#8217;re doing great! It was really impressive to see the mycelium really take over the rye in the bags. One of the bags got some bacteria in it so we need to throw it out. Unfortunately the Pleurotus in the coffee grounds didn&#8217;t do as well as the rye.<\/p>\n<div id=\"attachment_244\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0698.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-244\" class=\"size-medium wp-image-244\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0698-300x225.jpg\" alt=\"Inoculated bags\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0698-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0698-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0698-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-244\" class=\"wp-caption-text\">Inoculated bags<\/p><\/div>\n<div id=\"attachment_245\" style=\"width: 235px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0699.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-245\" class=\"size-medium wp-image-245\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0699-225x300.jpg\" alt=\"Pleurotus\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0699-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0699-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><p id=\"caption-attachment-245\" class=\"wp-caption-text\">Pleurotus<\/p><\/div>\n<div id=\"attachment_246\" style=\"width: 235px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0700.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-246\" class=\"size-medium wp-image-246\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0700-225x300.jpg\" alt=\"coffee grounds\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0700-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0700-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><p id=\"caption-attachment-246\" class=\"wp-caption-text\">coffee grounds<\/p><\/div>\n<div id=\"attachment_247\" style=\"width: 235px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0701.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-247\" class=\"size-medium wp-image-247\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0701-225x300.jpg\" alt=\"more rye bags\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0701-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0701-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><p id=\"caption-attachment-247\" class=\"wp-caption-text\">Trametes<\/p><\/div>\n<p>&#8211; Vanessa <strong>Subculturing<\/strong> 07\/17\/2013 We received a package from Fungi Perfecti with Stropharia. I subcultured it onto new MEA plates. I also did a tissue explant onto plates with another Pleurotus strain Professor Hibbett brought. -Vanessa <strong>Inoculating<\/strong> 07\/22\/2013 Today I inoculated 9 bags of rye, each with 1L of rye and 900 ml of water, with the Pleurotus from Fungi Perfecti that we had in a jug. The Stropharia culture on the plates is growing!<\/p>\n<div id=\"attachment_251\" style=\"width: 235px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0713.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-251\" class=\"size-medium wp-image-251\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0713-225x300.jpg\" alt=\"Stropharia cultures\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0713-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0713-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><p id=\"caption-attachment-251\" class=\"wp-caption-text\">Stropharia cultures<\/p><\/div>\n<p>-Vanessa <strong>\u00a0More Inoculating<\/strong> 07\/28\/2013 Today I inoculated six jars of rye (30g of rye and 40 ml of water) with the previous cultures of pleurotus ostreatus strain. &#8211; Vanessa <strong>Passive Samplers<\/strong> 07\/31\/2013 Jim Rice sent us information on what he did in the canal with the passive samplers. Here is a brief description he wrote for us.<\/p>\n<div>The passive sampler study uses thin sheets of a plastic polymer,\u00a0polyethylene (PE),\u00a0deployed at fixed sites in the canal over a period of four weeks, that are then collected for analysis. In the water, the samplers passively accumulate contaminant compounds that are quantified in a lab at the end of the deployment period. The amount of the accumulated contaminants in each sheet of polymer is used to derive an estimate of the amount of contaminant that is freely dissolved in the canal.<\/div>\n<div>This freely dissolved concentration is an important value because it is closely related to the amount of contaminant that is available for accumulation in fish or other organisms.<i> <\/i><\/div>\n<div><\/div>\n<div>Among other techniques used to determine the freely dissolved concentration of hydrophobic contaminants, passive samplers have the advantage of long-term equilibrium partitioning over the duration of their deployment, which is preferable in cases when a time-weighted average concentration is needed.<\/div>\n<div><\/div>\n<div>\n<div id=\"attachment_258\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1892.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-258\" class=\"size-medium wp-image-258\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1892-300x225.jpg\" alt=\"The sampler (test).\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1892-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1892-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1892-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-258\" class=\"wp-caption-text\">The sampler (test).<\/p><\/div>\n<div id=\"attachment_259\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1924.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-259\" class=\"size-medium wp-image-259\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1924-300x225.jpg\" alt=\"in the canal\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1924-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1924-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_1924-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-259\" class=\"wp-caption-text\">in the canal<\/p><\/div>\n<div id=\"attachment_260\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/Sampler-Suspended.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-260\" class=\"size-medium wp-image-260\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/Sampler-Suspended-300x225.jpg\" alt=\"on deployment day 07\/02\/2013\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/Sampler-Suspended-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/Sampler-Suspended-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/Sampler-Suspended-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-260\" class=\"wp-caption-text\">on deployment day 07\/02\/2013<\/p><\/div>\n<div id=\"attachment_261\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/Surface-Oil-between-Booms.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-261\" class=\"size-medium wp-image-261\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/Surface-Oil-between-Booms-300x225.jpg\" alt=\"Surface Oil between Booms\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/Surface-Oil-between-Booms-300x225.jpg 300w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/Surface-Oil-between-Booms-1024x768.jpg 1024w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/Surface-Oil-between-Booms-400x300.jpg 400w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-261\" class=\"wp-caption-text\">Surface Oil between Booms<\/p><\/div>\n<p><strong>\u00a0Inoculating 20 more bags<\/strong><\/div>\n<p>08\/02\/2013 Today I inoculated 8 bags with the new stropharia culture we got from Fungi Perfecti. I also Inoculated 12 bags with the Pleurotus Eryngii cultures.<\/p>\n<div id=\"attachment_255\" style=\"width: 235px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0724.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-255\" class=\"size-medium wp-image-255\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0724-225x300.jpg\" alt=\"Pleurotus Eryngii\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0724-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0724-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><p id=\"caption-attachment-255\" class=\"wp-caption-text\">Pleurotus Eryngii<\/p><\/div>\n<div id=\"attachment_256\" style=\"width: 235px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0725.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-256\" class=\"size-medium wp-image-256\" src=\"https:\/\/wordpress.clarku.edu\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0725-225x300.jpg\" alt=\"Stropharia\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0725-225x300.jpg 225w, https:\/\/wordpress.clarku.edu\/fisherville\/wp-content\/uploads\/sites\/246\/2013\/06\/IMG_0725-768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><p id=\"caption-attachment-256\" class=\"wp-caption-text\">Stropharia<\/p><\/div>\n<p>-Vanessa The Big Day 08\/07\/2013 Today was the big day! Professor Hibbett and I went to the Fisherville site. We took the 23 bags of mycelium inoculated with Irpex, Trametes, and Pleurotus. We joined Gene, his son Nick, and Max from John Todd Ecological Designs. First we cleaned the 23 bins with water. We added about 20 lbs of hard wood pellets (an oak and maple blend). Next we added about four gallons of water to each bin and let the pellets absorbs the water. It was amazing to see the pellets grow with water! We labeled the bins with a labeler that Nick had with the names of each of the fungi. We then gloved up and inoculated the bins with the bags of mycelium. We had to be careful and break apart the mycelium on the rye. It was a really interesting texture: slimy and but very cohesive. We spread out the mycelium in the wood chips as thoroughly as we could.We then carried the bins inside the trailer with air-conditioning and covered them up. The bins are now ready to be placed in the eco-machine.<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p>August 4, 2014 National Park Service Project Proposal Submitted \u00a0 This past week The Fisherville Redevelopment Company teamed up with the Town of Grafton to submit a proposal to National [&hellip;]<\/p>\n","protected":false},"author":330,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-39","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/pages\/39","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/users\/330"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/comments?post=39"}],"version-history":[{"count":0,"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/pages\/39\/revisions"}],"wp:attachment":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/media?parent=39"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}