{"id":346,"date":"2014-08-26T13:59:10","date_gmt":"2014-08-26T13:59:10","guid":{"rendered":"https:\/\/wordpress.clarku.edu\/fisherville\/?page_id=346"},"modified":"2014-08-26T14:11:30","modified_gmt":"2014-08-26T14:11:30","slug":"degradation-of-bunker-c-oil-by-white-rot-basidiomycetes","status":"publish","type":"page","link":"https:\/\/wordpress.clarku.edu\/fisherville\/projects\/degradation-of-bunker-c-oil-by-white-rot-basidiomycetes\/","title":{"rendered":"Degradation of Bunker C oil by white-rot basidiomycetes"},"content":{"rendered":"

This project was conducted by Darcy Young (now in\u00a0Amy Zanne’s lab<\/a>\u00a0at George Washington University) as part of her master\u2019s research in the\u00a0Hibbett lab<\/a>\u00a0of the\u00a0Biology Department at Clark University<\/a>, in collaboration with\u00a0James Rice<\/a>\u00a0at the\u00a0Brown University Superfund Lab<\/a>. The goals of this project were 1) to assess the ability of wood-decaying basidiomycetes to degrade hyrocarbons in Bunker C oil (the primary legacy pollutant at the Fisherville Mill site), and 2) to investigate shifts in gene expression in the presence of Bunker C oil.<\/p>\n

For this work, we used \u201cwhite rot\u201d fungi, which produce enzymes that are capable of degrading lignin, a complex polymer in woody plant cell walls that is resistant to microbial attack.<\/p>\n

\"Rarcy<\/a><\/p>\n

Darcy Young inoculating the mycoreactor at at the Fisherville site.<\/em><\/p>\n

\"Fungal<\/a><\/p>\n

Fungal cultures on sawdust with (right) and without (left) Bunker C oil.<\/em><\/p>\n

We grew four selected species (Irpex lacteus<\/i>,\u00a0Trichaptum biforme<\/i>,\u00a0Phlebia radiata<\/i>,\u00a0Trametes versicolor<\/i>, and\u00a0Pleurotus ostreatus<\/i>) in the presence of oil on sawdust media and assayed hydrocarbon degradation with gas chromatography-mass spectrometry (GC-MS). We also extracted RNA from cultures of a fifth species,\u00a0Punctularia strigoso-zonata<\/i>, grown with or without Bunker C oil present and assessed gene expression with RNAseq, taking advantage of the whole genome sequence of this species that has been generated by the US DOE\u00a0Joint Genome Institute<\/a>. GC-MS results indicate that the species we investigated were very effective at removing selected focal hyrocarbons, but transcriptome data were more ambiguous. A manuscript reporting this work is in preparation. This work was supported in part by a grant from the\u00a0Mosakowski Institute<\/a>\u00a0of Clark University.<\/p>\n

\n","protected":false},"excerpt":{"rendered":"

This project was conducted by Darcy Young (now in\u00a0Amy Zanne’s lab\u00a0at George Washington University) as part of her master\u2019s research in the\u00a0Hibbett lab\u00a0of the\u00a0Biology Department at Clark University, in collaboration […]<\/p>\n","protected":false},"author":416,"featured_media":0,"parent":35,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-346","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/pages\/346","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/users\/416"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/comments?post=346"}],"version-history":[{"count":0,"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/pages\/346\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/pages\/35"}],"wp:attachment":[{"href":"https:\/\/wordpress.clarku.edu\/fisherville\/wp-json\/wp\/v2\/media?parent=346"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}