{"id":125,"date":"2013-11-27T15:02:49","date_gmt":"2013-11-27T15:02:49","guid":{"rendered":"https:\/\/wordpress.clarku.edu\/dlarochelle\/?page_id=125"},"modified":"2023-12-13T20:31:59","modified_gmt":"2023-12-13T20:31:59","slug":"protocols","status":"publish","type":"page","link":"https:\/\/wordpress.clarku.edu\/dlarochelle\/resources\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"

Protocols for working with proteins<\/span>:
\n<\/strong>Immunoprecipitations<\/a>
\n<\/a><\/strong>SDS_Page_Gels
\n<\/a>Western Blot<\/a>
\nSilver_Staining
\n<\/a>Protein_Samples
\n<\/a>Coomassie Staining<\/h4>\n

Microscopy protocols<\/span>:
\n<\/strong>Dapi_Staining
\n<\/a>Indirect_Immunoflourescence<\/a>
\nFixing Cells<\/h4>\n

Molecular Protocols<\/span>:<\/strong>
\nPolymerase Chain Reactions<\/a>
\nMiniprep<\/a>
\nSequencing<\/a>
\nSouthern Blot<\/a>
\nNorthern_Blot<\/a>
\nRNA_Isolation<\/a>
\nRNA_Agarose_Gel<\/a>
\nGenomic_DNA_Isolation
\n<\/a>Transformation_of_E_coli<\/a>
\nTransfection_of_Dh5_specific_E_coli<\/a>
\nCaCl2_Competent_Cells<\/a><\/h4>\n

Cell Protocols<\/span>:
\n<\/strong>Cell_Culture<\/a>
\n<\/strong>Transfection_into_Dictyostelium<\/a>
\nCentrosome_Isolation
\n<\/a>Passing Cells
\nCentrosome, Nuclei, and Enrichment-Purifying<\/h4>\n

Recipes for Protocols<\/span>:<\/strong>
\nRecipes for protein reagents<\/a>
\nRecipes_for_microscopy_reagents
\n<\/a>Recipes_for_molecular_reagents
\n<\/a>Recipes_for_cell_reagents<\/a>
\nRecipes_for_general_lab_reagents<\/a><\/h4>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Protocols for working with proteins: Immunoprecipitations SDS_Page_Gels Western Blot Silver_Staining Protein_Samples Coomassie Staining Microscopy protocols: Dapi_Staining Indirect_Immunoflourescence Fixing Cells Molecular Protocols: Polymerase Chain Reactions Miniprep Sequencing Southern Blot Northern_Blot RNA_Isolation RNA_Agarose_Gel Genomic_DNA_Isolation Transformation_of_E_coli Transfection_of_Dh5_specific_E_coli CaCl2_Competent_Cells Cell Protocols: Cell_Culture Transfection_into_Dictyostelium Centrosome_Isolation … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":323,"featured_media":0,"parent":24,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-125","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/pages\/125","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/users\/323"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/comments?post=125"}],"version-history":[{"count":0,"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/pages\/125\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/pages\/24"}],"wp:attachment":[{"href":"https:\/\/wordpress.clarku.edu\/dlarochelle\/wp-json\/wp\/v2\/media?parent=125"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}