Stock solution is 10 mg\/mL and is made with deionized water.\u00a0 Store the stock solution at 4\u00b0C.<\/p>\n
Add 4 \u00b5L of the stock solution to 100 mL of dissolved agarose.<\/p>\n
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There are several solutions that can be used.<\/p>\n
1) 0.25 (w\/v) % bromophenol blue; 0.25% (w\/v) xylene cyanol; 30% glycerol.<\/p>\n
Make a 10 mL solution and store at either 4\u00b0C or -20\u00b0C.<\/p>\n
[This is the answer to the question.]<\/p>\n
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This is a common solution used in molecular biology and is the abbreviation for a 10 mM Tris, 1 mM EDTA solution.\u00a0 The pH of the solution is usually 8.0.<\/p>\n
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Add 186.1 g of disodium EDTA (always double check molecular weight and calculations) to 800 mL of dH2O.\u00a0 Adjust the pH to 8.0 with NaOH (ca. 20 g of NaOH pellets).\u00a0 Adjust volume to 1 L.\u00a0 Dispense into aliquots and autoclave.<\/p>\n
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Isopropylthio-\u00df-D-galactoside (m.w. = 238.3).\u00a0 Make a solution of IPTG by dissolving 2 g of IPTG in 8 mL of dH2O.\u00a0 Adjust volume to 10 mL and sterilize by filtration through a 0.22 \u00b5 disposable filter.\u00a0 Dispense the solution in 1 mL aliquots and store them at -20\u00b0 C.<\/p>\n
Ethidium bromide solutions for gel staining Stock solution is 10 mg\/mL and is made with deionized water.\u00a0 Store the stock solution at 4\u00b0C. Add 4 \u00b5L of the stock solution to 100 mL of dissolved agarose. DNA Sample Loading …<\/p>\n