{"id":248,"date":"2012-04-10T16:33:43","date_gmt":"2012-04-10T16:33:43","guid":{"rendered":"https:\/\/wordpress.clarku.edu\/debrobertson\/?page_id=248"},"modified":"2012-05-23T17:19:08","modified_gmt":"2012-05-23T17:19:08","slug":"bradford-microassay","status":"publish","type":"page","link":"https:\/\/wordpress.clarku.edu\/debrobertson\/laboratory-protocols\/bradford-microassay\/","title":{"rendered":"BRADFORD MICROASSAY"},"content":{"rendered":"<ol>\n<li>Prepare a standard curve of five to six concentrations of a protein standard, which is representative of the protein solution to be tested. Usually 0 &#8211; 10 ug of protein adjusted to 800 uL with water or sample buffer.<\/li>\n<li>Pipet 10 uL each sample into a tube and adjust volume to 800 uL (NOTE: the volume of the unknown can be adjusted to as much as 800 uL). Samples are usually assayed in duplicate or triplicate.<\/li>\n<li>Add 200 uL of the dye reagent (DO NOT DILUTE). Vortex each tube.<\/li>\n<li>Incubate at room temperatre for at least 5 minutes. Absorbance will increase over time; samples should incubate at room temperature for no more than 1 hour.<\/li>\n<li>Measure absorbance at 595 nm<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Prepare a standard curve of five to six concentrations of a protein standard, which is representative of the protein solution to be tested. Usually 0 &#8211; 10 ug of protein adjusted to 800 uL with water or sample buffer. Pipet &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"more-link\" href=\"https:\/\/wordpress.clarku.edu\/debrobertson\/laboratory-protocols\/bradford-microassay\/\"> <span class=\"screen-reader-text\">BRADFORD MICROASSAY<\/span> Read More &raquo;<\/a><\/p>\n","protected":false},"author":72,"featured_media":0,"parent":8,"menu_order":55,"comment_status":"closed","ping_status":"closed","template":"onecolumn-page.php","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-248","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages\/248","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/users\/72"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/comments?post=248"}],"version-history":[{"count":0,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages\/248\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages\/8"}],"wp:attachment":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/media?parent=248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}