{"id":235,"date":"2012-04-10T16:19:58","date_gmt":"2012-04-10T16:19:58","guid":{"rendered":"https:\/\/wordpress.clarku.edu\/debrobertson\/?page_id=235"},"modified":"2012-05-23T17:19:33","modified_gmt":"2012-05-23T17:19:33","slug":"recipes-for-mini-page-gels","status":"publish","type":"page","link":"https:\/\/wordpress.clarku.edu\/debrobertson\/laboratory-protocols\/recipes-for-mini-page-gels\/","title":{"rendered":"RECIPES FOR MINI-PAGE GELS"},"content":{"rendered":"\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
TYPE OF GEL: DISCONTINUOUS SDS-PAGE USING OWL PENGUIN SYSTEM<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
RESOLVING GEL:\u00a0(variable %T, 0.375 M Tris-HCl, pH 8.8)<\/span><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Volumes are in mL unless noted; total volume is 10 mL, just enough for two mini-gels.\u00a0 You may choose to make 15 mL.<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\nFinal Acrylamide Percentage<\/strong><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Stock Solution<\/td>\n5<\/td>\n7.5<\/td>\n10<\/td>\n12.5<\/td>\n15<\/td>\n17.5<\/td>\n20<\/td>\n<\/td>\n<\/tr>\n
Acrylamide-bisacrylamide<\/td>\n1.25<\/td>\n1.88<\/td>\n2.50<\/td>\n3.13<\/td>\n3.75<\/td>\n4.38<\/td>\n5.00<\/td>\n<\/td>\n<\/tr>\n
(40% T<\/span>, 3.33% C)<\/span><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Distilled water<\/td>\n6.10<\/td>\n5.47<\/td>\n4.85<\/td>\n4.22<\/td>\n3.60<\/td>\n2.97<\/td>\n2.35<\/td>\n<\/td>\n<\/tr>\n
10% SDS<\/td>\n0.1<\/td>\n0.1<\/td>\n0.1<\/td>\n0.1<\/td>\n0.1<\/td>\n0.1<\/td>\n0.1<\/td>\n<\/td>\n<\/tr>\n
1.5 M Tris-HCl, pH 8.8<\/td>\n2.5<\/td>\n2.5<\/td>\n2.5<\/td>\n2.5<\/td>\n2.5<\/td>\n2.5<\/td>\n2.5<\/td>\n<\/td>\n<\/tr>\n
10% (w\/v) Ammonium persulfate<\/td>\n50 \u00b5L<\/td>\n50 \u00b5L<\/td>\n50 \u00b5L<\/td>\n50 \u00b5L<\/td>\n50 \u00b5L<\/td>\n50 \u00b5L<\/td>\n50 \u00b5L<\/td>\n<\/td>\n<\/tr>\n
(make fresh regularly)<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Temed<\/td>\n5 \u00b5L<\/td>\n5 \u00b5L<\/td>\n5 \u00b5L<\/td>\n5 \u00b5L<\/td>\n5 \u00b5L<\/td>\n5 \u00b5L<\/td>\n5 \u00b5L<\/td>\n<\/td>\n<\/tr>\n
(after degassing)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
STACKING GEL:\u00a0(4%T, 0.125 M Tris-HCl, pH 6.8)<\/span><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Volumes are in mL unless noted; total volume is 10 mL enough for two mini-gels<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
Stock Solution<\/td>\n10 mL<\/td>\n5 mL<\/td>\n2.5 mL<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Acrylamide-bisacrylamide<\/td>\n1<\/td>\n0.5<\/td>\n0.25<\/td>\n<\/td>\n<\/tr>\n
(40% T, 3.33% C)<\/td>\n<\/td>\n<\/tr>\n
Distilled water<\/td>\n6.35<\/td>\n3.17<\/td>\n1.59<\/td>\n<\/td>\n<\/tr>\n
0.5 M Tris-HCl, pH 6.8<\/td>\n2.50<\/td>\n1.25<\/td>\n0.63<\/td>\n<\/td>\n<\/tr>\n
10% SDS<\/td>\n0.1<\/td>\n0.05<\/td>\n0.025<\/td>\n<\/td>\n<\/tr>\n
10% (w\/v) Ammonium persulfate<\/td>\n50 \u00b5L<\/td>\n25 \u00b5L<\/td>\n12.5 \u00b5L<\/td>\n<\/td>\n<\/tr>\n
Temed<\/td>\n10 \u00b5L<\/td>\n5 \u00b5L<\/td>\n2.5 \u00b5L<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

TYPE OF GEL: DISCONTINUOUS SDS-PAGE USING OWL PENGUIN SYSTEM RESOLVING GEL:\u00a0(variable %T, 0.375 M Tris-HCl, pH 8.8) Volumes are in mL unless noted; total volume is 10 mL, just enough for two mini-gels.\u00a0 You may choose to make 15 mL. …<\/p>\n

RECIPES FOR MINI-PAGE GELS<\/span> Read More »<\/a><\/p>\n","protected":false},"author":72,"featured_media":0,"parent":8,"menu_order":35,"comment_status":"closed","ping_status":"closed","template":"onecolumn-page.php","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-235","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages\/235","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/users\/72"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/comments?post=235"}],"version-history":[{"count":0,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages\/235\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/pages\/8"}],"wp:attachment":[{"href":"https:\/\/wordpress.clarku.edu\/debrobertson\/wp-json\/wp\/v2\/media?parent=235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}