Publications

Google Scholar

Pubmed

2017
RBR E3 Ubiquitin Ligases.
Beasley, S.A., Wang, Y. and Spratt, D.E. (2017) Encyclopedia of Signaling Molecules 2nd Ed. in press.

2016
Generation of phospho-ubiquitin variants by orthogonal translation reveals codon skipping.
George, S., Aguirre, J.D., Spratt, D.E., Bi, Y., Jeffery, M., Shaw, G.S., and O’Donoghue, P. (2016) FEBS Lett. 590, 1530-1542.

Suramin inhibits cullin-RING E3 ubiquitin ligases.
Wu, K., Chong, R.A., Yu, Q., Bai, J., Spratt, D.E., Ching, K., Lee, C., Miao, H., Tappin, I., Hurwitz, J., Zheng, N., Shaw, G.S., Sun, Y., Felsenfeld, D.P., Sanchez, R., Zheng, J.N., and Pan, Z.-Q. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E2011-2018.

2015
Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis.
Kumar, A., Aguirre, J.D., Condos, T.E.C., Martinez-Torres, R.J., Chaugule, V.K., Toth, R., Sundaramoorthy, R., Mercier, P., Knebel, A., Spratt, D.E., Barber, K.R., Shaw, G.S., and Walden, H. (2015) EMBO J. 34, 2506-2521.

2014
RBR E3 ubiquitin ligases: new structures, new insights, new questions.
Spratt, D.E., Walden, H., and Shaw, G.S. (2014, invited review) Biochem. J. 458, 421-437.

Pivotal role for the ubiquitin Y59-E51 loop in lysine-48 polyubiquitination.
Chong, R.A., Wu, K., Spratt, D.E., Yang, Y., Lee, C., Nayak, J., Xu, M., Elkholi, R., Li, J., Brown, B.D., Chipuk, J.E., Chen, Z., Sanchez, R., Shaw, G.S., Huang, L., and Pan, Z.-Q. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, 8434-8439.

A snapshot at ubiquitin chain elongation: Lysine 48-tetra-ubiquitin slows down ubiquitination.
Kovacev, J., Wu, K., Spratt, D.E., Chong, R.A., Nayak, J., Shaw, G.S., and Pan, Z.-Q. (2014) J. Biol. Chem. 289, 7068-7081.

2013
A molecular explanation for the recessive nature of parkin-linked Parkinson’s disease.
Spratt, D.E., Martinez-Torres, R.J., Noh, Y.J., Mercier, P., Manczyk, N., Barber, K.R., Aguirre, J.D., Burchell, L., Purkiss, A., Walden, H., and Shaw, G.S. (2013) Nat. Commun. 4, 1983. 
***Highlighted on the Michael J. Fox Foundation for Parkinson’s Research Website (August 1, 2013).

Structure of the HHARI catalytic domain shows glimpses of a HECT E3 ligase. Spratt, D.E., Mercier, P., and Shaw, G.S. (2013) PLoS ONE 8, e74047.

2012
Selective recruitment of an E2~ubiquitin complex by an E3 ubiquitin ligase.
Spratt, D.E., Wu, K., Kovacev, J., Pan, Z.-Q., and Shaw, G.S. (2012) J. Biol. Chem. 287, 17374-17385.

Structure and dynamics of calmodulin (CaM) bound to nitric oxide synthase peptides: effects of a phosphomimetic calmodulin mutation.
Piazza, M., Futrega, K., Spratt, D.E., Dieckmann, T., and Guillemette, J.G. (2012) Biochemistry 51, 3651-3661.

2011
Association of the disordered C-terminus of CDC34 with a catalytically-bound ubiquitin. 
Spratt, D.E., and Shaw, G.S. (2011) J. Mol. Biol. 407, 425-438.

Expression and purification of an isotopically labeled aggregation prone iNOS CaM binding protein for use in NMR studies.
Piazza, M., Duangkham, Y., Spratt, D.E., Dieckmann, T., and Guillemette, J.G. (2011) J. Label. Compd. Radiopharm. 54, 657-663.

Mapping the binding and calmodulin-dependent activation of nitric oxide synthase isozymes.
Spratt, D.E., Duangkham, Y., Taiakina, V., and Guillemette, J.G. (2011) The Open Nitric Oxide Journal 3, 16-24.

2010
Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins.
Marlatt, N.M., Spratt, D.E., and Shaw, G.S. (2010) Protein Expr. Purif. 73, 58-64.

2009
Intraprotein electron transfer in inducible nitric oxide synthase holoenzyme.
Feng, C., Dupont, A.L., Nahm, N.J., Spratt, D.E., Weinberg, J.B., Guillemette, J.G., Salerno, J.C., Tollin, G., and Ghosh, D.K. (2009) J. Biol. Inorg. Chem. 14, 133-142.

2008
FRET conformational analysis of calmodulin binding to nitric oxide synthase peptides and enzymes.
Spratt, D.E., Taiakina, V., Palmer, M., and Guillemette, J.G. (2008) Biochemistry 47, 12006-12017.

Regulation of mammalian nitric oxide synthases by electrostatic interactions in the linker region of calmodulin.
Spratt, D.E., Israel, O., Taiakina, V., and Guillemette, J.G. (2008) Biochim. Biophys. Acta. 1784, 2065-2070.

2007
Calcium-deficient calmodulin binding and activation of neuronal and inducible nitric oxide synthases.
Spratt, D.E., Taiakina, V., and Guillemette, J.G. (2007) Biochim. Biophys. Acta. 1774, 1351-1358.

Differential binding of calmodulin domains to constitutive and inducible nitric oxide synthase enzymes.
Spratt, D.E., Taiakina, V., Palmer, M., and Guillemette, J.G. (2007)Biochemistry 46, 8288-8300.

2006
Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs. Spratt, D.E., Newman, E., Mosher, J., Ghosh, D.K., Salerno, J.C., and Guillemette, J.G. FEBS J. 273, 1759-1771.

Selective labeling of selenomethionine residues in proteins with a fluorescent derivative of benzyl bromide.
Lang, S., Spratt, D.E., Guillemette, J.G., and Palmer, M. (2006) Anal. Biochem. 359, 253-258.

2005
Dual-targeted labeling of proteins using cysteine and selenomethionine residues.
Lang, S., Spratt, D.E., Guillemette, J.G., and Palmer, M. (2005) Anal. Biochem. 342, 271-279.

2004
Differential activation of nitric-oxide synthase isozymes by calmodulin-troponin C chimeras.
Newman, E., Spratt, D.E., Mosher, J., Cheyne, B., Montgomery, H.J., Wilson, D.L., Weinburg, J.B., Smith, S.M.E., Salerno, J.C., Ghosh, D.K., and Guillemette, J.G. (2004) J. Biol. Chem. 279, 33547-33557.