Equipment

  • AKTA pure 25L (GE Healthcare) – FPLC for protein purification
  • Numerous affinity, HIC, IEX, and gel filtration columns (GE Healthcare) – for protein purification
  • Emulsiflex C5 Homogenizer (Avestin) – for high efficiency lysis of E. coli
  • Sorvall Lynx 4000 Centrifuge (Thermo) – routine centrifugation to harvest bacteria and/or purify proteins
  • New Brunswick Incubator Shakers I26R and New Brunswick Excella E24 shakers (Eppendorf) – for bacteria growth and protein overexpression
  • T-100 PCR machine (Bio-Rad) – for cloning and mutagenesis reactions
  • UV/Vis Spectrophotometers (Shimadzu)
  • -80C Isotemp Freezer (Fisher Sci.)
  • Tetra-Pak SDS-PAGE apparatus (Bio-Rad) – for SDS-PAGE
  • pH meters (VWR)
  • Vortexes (Fisher Sci)

Access other pieces of equipment @ Clark University including two Varian INOVA 600 MHz NMR Spectrometer (4 complete channel plus deuterium decoupling accessory, HCN probe with XYZ axial gradients)